gradient: 3% ACN/min
packed with 3um C18 particles.
Average peak width at 2σ: 2 s
Sample: Five proteins tryptic digest, 1 fmol/uL
Injection: 0-5 min, 10 min @ 3uL/min on a 20mmLx50 umID 5um C18 trap
NanoSeparations is a young, dynamic and innovative
company focussing primarily on nano-scale liquid chromatography (nLC)
for proteome analysis and related areas. Our primary goal is
establishing refinements in LCMS technologies for sensitive,
selective and comprehensive complex peptide sample analysis.
In 2003, NanoSeparations introduced for the first time a truly
25 umID nanoflow column together with
an unique, low cost
column switching system that fully preserves the chromatographic
integrity, speed and inherent gain in sensitivity. This system converts any conventional LCMS
instrument into a high performance nano-scale system.
Also in 2004,
NanoSeparations introduced the extended length column up to 100cm
length and true 2D
separation systems including a versatile, selective on-line system for
enrichment of phosphopeptides at the sub femtomole
Nanoseparations produces highest quality nanoflow columns packed with 5, 3
1.8 um particles in column formats of 200 to 25 um ID and various column
up to 1 meter